Nuclear magnetic resonance and surface-assisted laser desorption/ionization mass spectrometry-based metabolome profiling of urine samples from kidney cancer patients

Kidney cancer is one of the most frequently diagnosed cancers urinary tract in world. Despite significant advances kidney treatment, no urine specific biomarker currently used to guide therapeutic interventions. In an effort address this knowledge gap, metabolic profiling samples from 50 patients with and healthy volunteers was undertaken using high-resolution proton nuclear magnetic resonance spectroscopy (1H NMR) silver-109 nanoparticle enhanced steel target laser desorption/ionization mass spectrometry (109AgNPET LDI MS). Twelve potential biomarkers were identified quantified one-dimensional (1D) 1H NMR metabolomics. Seven spectral features which differed significantly abundance (p < 0.05) between also detected 109AgNPET-based (LDI This work provides a framework expand discovery that could be as useful diagnostic or prognostic progression